Additional Selective and Differential Cell Culture Media
NEOGEN HC Agar Base, 500 g
HC Agar Base is used with Tween 80 (Polysorbate 80) for the enumeration of molds in cosmetics in a laboratory setting. HC Agar Base is not intended for use in the diagnosis of disease or other conditions in humans.Methods for isolating molds from cosmetic products require incubation for 5 - 7 days using traditional agar media. In 1986, Mead and O’Neill described a new medium, HC Agar, for enumerating molds in cosmetic products. The formulation of HC Agar decreased incubation time to 3 days at 27.5 ± 0.5°C for molds. HC Agar Base, based on the HC Agar formula of Mead and O’Neill, is supplemented with Tween 80 (Polysorbate 80) to prepare HC Agar.For more information visit https://www.neogen.com/solutions/Microbiology/hc-agar-base
NEOGEN Rappaport-Vassiliadis Medium with Soya (RVS) (ISO), 5 kg
Rappaport-Vassiliadis Medium with Soya is used as a selective enrichment medium for the detection of Salmonella spp. in food, animal feed and in environmental samples from the food production area as described in ISO 6579-1:2017. RVS is not intended for use in the diagnosis of disease or other conditions in humans.Rappaport et al. originally described the broth, which was later modified by Vassiliadis et al. and optimized by van Schothorst et al. Enzymatic digest of soya provides a source of nitrogen and amino acids and sodium chloride provides osmotic pressure. The potassium phosphate components act as a buffer. Magnesium chloride and malachite green act as selective agents against non-target microorganisms. The formulation is very hygroscopic and will produce a slight exothermic reaction when mixed with water.For more information visit https://www.neogen.com/solutions/Microbiology/rappaport-vassiliadis-medium-with-soya
NEOGEN Tryptic Soy Broth, Modified with 20 mg Novobiocin & Acid Digest Casein, 500 g
TSB, Modified with 20mg Novobiocin & Acid Digest of Casein is used for the selective enrichment of enterohemorrhagic E. coli in foods in a laboratory setting. TSB Modified w/20 mg Novobiocin & Acid Digest of Casein is not intended for use in the diagnosis of disease or other conditions in humans. The first major outbreak of E. coli O157:H7 was in 1982, and traced to contaminated hamburgers. Other known sources of infection include sprouts, lettuce, salami, unpasteurized milk, juice and/or swimming in or drinking contaminated water. TSB, Modified w/20 mg Novobiocin & Acid Digest of Casein is used to enrich food samples suspected of having low levels of EHEC during pathogen testing. This formulation conforms to the USDA/FSIS Microbiology Laboratory Guidebook.For more information visit https://www.neogen.com/solutions/Microbiology/tryptic-soy-broth-modified-with-20-mg-novobiocin-and-acid-digest-casein
NEOGEN Xylose Lysine Deoxycholate (XLD) Agar (ISO), 5 kg
Xylose Lysine Deoxycholate (XLD) Agar (ISO) is a selective agar for the detection of Salmonella spp. in food, animal feed and in environmental samples from the food production area as described in ISO 6579-1:2017. Xylose Lysine Deoxycholate (XLD) Agar (ISO) is not intended for use in the diagnosis of disease or other conditions in humans. A selective, diagnostic agar for the detection of Salmonella spp. in food, animal feed and in environmental samples from the food production area as described in ISO 6579-1:2017. Originally formulated by Taylor to differentiate enteric pathogens, the agar is widely used as the preferred differential medium for Salmonella spp. The medium is void of peptones but instead uses yeast extract as a carbon, nitrogen and vitamin source and xylose, lactose and sucrose are fermentable carbohydrates.For more information visit https://www.neogen.com/solutions/Microbiology/xylose-lysine-deoxycholate-xld-agar-iso
NEOGEN GC Agar, 5 kg
GC Agar is used with lysed blood and other supplements for the isolation and cultivation of Neisseria gonorrhoeae and other fastidious organisms. GC Agar is not intended for use in the diagnosis of disease or other conditions in humans.A nutritious agar base described by Thayer and Martin for the isolation of Neisseria gonorrhoeae. The rich peptone mixture is enhanced by the use of corn starch to absorb toxic metabolites and a buffering system is used to maintain neutral pH. The medium is made selective by the use of various antibiotic cocktails. Thayer and Martin originally recommended the use of vancomycin, colistin and nystatin and the addition of trimethoprim which is useful in preventing the swarming of proteus. More recently the emergence of vancomycin sensitive gonococci has made the selective agents (lincomycin, colistin, amphotericin, trimethoprim (X070 LCAT) the combination of choice.For more information visit https://www.neogen.com/solutions/Microbiology/gc-agar
NEOGEN GC Agar, 500 g
GC Agar is used with lysed blood and other supplements for the isolation and cultivation of Neisseria gonorrhoeae and other fastidious organisms. GC Agar is not intended for use in the diagnosis of disease or other conditions in humans.A nutritious agar base described by Thayer and Martin for the isolation of Neisseria gonorrhoeae. The rich peptone mixture is enhanced by the use of corn starch to absorb toxic metabolites and a buffering system is used to maintain neutral pH. The medium is made selective by the use of various antibiotic cocktails. Thayer and Martin originally recommended the use of vancomycin, colistin and nystatin and the addition of trimethoprim which is useful in preventing the swarming of proteus. More recently the emergence of vancomycin sensitive gonococci has made the selective agents (lincomycin, colistin, amphotericin, trimethoprim (X070 LCAT) the combination of choice.For more information visit https://www.neogen.com/solutions/Microbiology/gc-agar
NEOGEN Fastidious Anaerobe Agar, 10 kg
Fastidious Anaerobe Agar is used for the cultivation of anaerobic microorganisms and is not intended for use in the diagnosis of disease or other conditions in humans. A primary isolation medium capable of growing most clinically significant anaerobes. Developed by Lab M (Neogen Corporation), comparisons have shown this medium to be superior to other formulations as a primary isolation medium for fastidious organisms. The peptones included have been chosen for maximum growth stimulation. Starch and sodium bicarbonate act as de-toxification agents while hemin encourages pigment production in Porphyromonas melaninogenicus. Specific growth promoting agents are Cysteine for Fusobacterium necrophorum, Propionibacterium acne and Bacteriodes fragilis, arginine for Eubacterium spp. soluble pyrophosphate for Porph. gingivalis and Porph. asaccharolyticus.For more information visit https://www.neogen.com/solutions/Microbiology/fastidious-anaerobe-agar
NEOGEN Cystine Lactose Electrolyte Deficient (CLED) Agar, 10 kg
Cystine Lactose Electrolyte Deficient (CLED) Agar is used for the differentiation and enumeration of microorganisms in urine. A medium for urine culture where the absence of electrolytes inhibits the swarming of Proteus spp. Cystine is added for the benefit of those organisms which have a specific cystine requirement. Differentiation of lactose and non-lactose fermenters is achieved using bromothymol blue as pH indicator. This medium supports the growth of Streptococcus pyogenes and most other fastidious organisms that do not require blood. Formula:Balanced Peptone - 4.0 g/LBeef Extract - 3.0 g/LTryptone - 4.0 g/LLactose - 10.0 g/LL-Cystine - 0.128 g/LBromothymol Blue - 0.02 g/LAgar - 15.0 g/LFor more information visit https://www.neogen.com/solutions/Microbiology/cystine-lactose-electrolyte-deficient-agar
NEOGEN Tryptone Soy Agar, 5 kg
Tryptone Soy Agar (TSA) is a general-purpose agar which will support the growth of a wide range of micro-organisms, and is not intended for use in the diagnosis of disease or other conditions in humans.The medium can be used for phage typing, colicine typing and for testing the X and V factor requirements of Haemophilus spp.Formula:Tryptone - 15.0 g/LSoy Peptone - 0.5 g/LSodium Chloride - 5.0 g/LAgar - 12.0 g/LFor more information visit https://www.neogen.com/solutions/Microbiology/tryptone-soy-agar
Biolegend Recombinant Human E-Cadherin-F
Recombinant Human E-Cadherin-Fc Chimera (carrier-free); Reactivity: Human; Apps: BA; Size: 100 μg
Promocell GmbH PromoCell Supplement Mix / Small Airway Epithelial Cell Growth Medium
PromoCell Airway Epithelial Cell Growth Medium is a serum-free medium optimized for the in vitro cultivation of epithelial cells from large air passages.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for equine, porcine, murine, bat and rat airway epithelial cells.To view a list of references where this medium was used with other species/cell types please check manufacturer homepage.| Media supplement | Final supplement concentration|| Bovine Pituitary Extract | 0.004 ml / ml || Epidermal Growth Factor (recombinant human) | 10 ng / ml || Insulin (recombinant human) | 5 μg / ml || Hydrocortisone | 0.5 μg / ml || Epinephrine | 0.5 μg / ml || Triiodo-L-thyronine | 6.7 ng / ml || Transferrin (recombinant human) | 10 μg / ml || Retinoic Acid | 0.1 ng / ml |
Promocell GmbH PromoCell Supplement Pack / Smooth Muscle Cell Growth Medium 2 Contains: C-30222, C-31010, C-37310, C-30320,
The PromoCell Smooth Muscle Cell Growth Medium 2 is a low-serum (5% V/V) medium developed to establish and maintain smooth muscle cell cultures from blood vessels and hollow organs.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for bovine, porcine, murine, dog, and rat smooth muscle cells.Components:* Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMix* Growth Medium Kit: Includes Basal Medium and SupplementPack* SupplementMix: Contains all media supplements premixed in one vial* SupplementPack: Contains all media supplements as individual vials| Media supplement | Final supplement concentration || Fetal Calf Serum | 0.05 ml / ml || Epidermal Growth Factor (recombinant human) | 0.5 ng / ml || Basic Fibroblast Growth Factor (recombinant human) | 2 ng / ml || Insulin (recombinant human) | 5 μg / ml |
Promocell GmbH PromoCell DC Base Medium 250 ml, with C-39853 attached to flask
The PromoCell Dendritic Cell (DC) Generation Medium has been developed for the easy and efficient generation of immature as well as fully mature myeloid Dendritic Cells from peripheral blood monocytes. The DC Base Medium is a user-customizable versions of the DC Generation Media that does not include cytokines.PromoCell DC Generation Medium allows for the generation of DC from cryopreserved as well as freshly isolated cells. In the latter case, the Monocyte Attachment Medium is also needed.PromoCell DC Media consist of a bottle of Basal Medium and one vial of SupplementMix. Adding the SupplementMix to the Basal Medium results in the complete Medium. Indeed, all DC Media must be supplemented with additional cytokines. The cytokines are included with the DC Generation Media but not with the DC Base Media.