Additional Selective and Differential Cell Culture Media
NEOGEN Buffered Listeria Enrichment Broth Base, 10 kg
Buffered Listeria Enrichment Broth Base is used with supplements for selective enrichment of Listeria spp. in foods and is not intended for use in the diagnosis of human disease.Listeria monocytogenes, described first in 1926 by Murray, Webb, and Swann, is an extensive problem in public health and food industries. This organism has the ability to cause human illness and death, particularly in immunocompromised individuals and pregnant women. Epidemiological evidence from outbreaks of listeriosis has indicated that the principle route of transmission is via consumption of foodstuffs contaminated with Listeria monocytogenes. Implicated vehicles of transmission include turkey, frankfurters, coleslaw, pasteurized milk, Mexican style cheese, and pate.For more information visit https://www.neogen.com/solutions/Microbiology/buffered-listeria-enrichment-broth-base
NEOGEN LESS Plus Medium Irradiated, 10 kg
Listeria monocytogenes, described first in 1926 by Murray, Webb and Swann, is an extensive problem in public health and food industries. This organism has the ability to cause human illness and death, particularly in immunocompromised individuals and pregnant women. Epidemiological evidence from outbreaks of listeriosis indicates the principle route of transmission is via the consumption of foods contaminated with Listeria monocytogenes. Implicated vehicles of transmission include meat, eggs, chicken, vegetables, and dairy products. Listeria spp. are ubiquitous in nature, present in a wide range of unprocessed foods and in soil, sewage, and river water. Certain strains of Listeria spp. are able to survive the manufacturing and ripening processes in dairy products.For more information visit https://www.neogen.com/solutions/Microbiology/less-plus-medium
NEOGEN Slanetz and Bartley Agar, 5 kg
Slanetz and Bartley Medium is used for the enumeration of enterococci from water samples as described in ISO 7899-2, and is not intended for use in the diagnosis of disease or other conditions in humans. This medium was originally described by Slanetz and Bartley for the enumeration of enterococci from water samples using a membrane filtration technique, but it may also be used as a spread plate for the examination of other sample types. Enterococci reduce tetrazolium chloride to the insoluble red dye formazan, producing colonies which are dark red or maroon on the surface of the membrane or agar. This reaction is not exclusive to enterococci, and the count at this stage should be considered presumptive. Colonies may be confirmed as enterococci by demonstrating aesculin hydrolysis using Kanamycin Aesculin Azide Agar (NCM0198).For more information visit https://www.neogen.com/solutions/Microbiology/slanetz-bartley-medium
NEOGEN Beef Extract Powder, 10 kg
Beef Extract Powder is a dehydrated extract of bovine tissue for use in preparing microbiological culture media in a laboratory setting. Beef Extract Powder is not intended for use in the diagnosis of disease or other conditions in humans.Beef Extract Powder is prepared and standardized for use in microbiological culture media, where it is generally used to replace infusion of meat. Culture media containing Beef Extract Powder are recommended for use in bacteriological examination of water, milk, and other materials, where uniform composition of media is important. Beef Extract Powder is relied upon for biochemical studies, particularly fermentation reactions because of its independence from fermentable substances. Several media containing Beef Extract Powder are recommended in standard methods for multiple application.For more information visit https://www.neogen.com/solutions/Microbiology/beef-extract-powder
NEOGEN Rose Bengal Chloramphenicol Agar, 500 g
Rose Bengal Chloramphenicol Agar is used for the selective isolation and enumeration of yeasts and molds from foods. Rose Bengal Chloramphenicol Agar is not intended for use in the diagnosis of disease or other conditions in humans. Rose Bengal Chloramphenicol Agar Base is a selective medium for the enumeration of fungi. In 1944, Smith and Dawson used Rose Bengal for the selective isolation of fungi from soil samples. This formula was prepared with a neutral pH, deviating from the usual acidified medium. Several investigations have found a neutral pH with the addition of a selective agent have been successful in supporting fungal growth and restricting bacterial growth. Rose Bengal Agar pre-supplemented with Chloramphenicol is a modification of the Rose Bengal Chlortetracycline Agar formula of Jarvis.For more information visit https://www.neogen.com/solutions/Microbiology/rose-bengal-chloramphenicol-agar
NEOGEN Tryptic Soy Broth (Soybean-Casein Digest Broth), 10 kg
Tryptic Soy Broth (Soybean-Casein Digest Broth) is used for the cultivation of a wide variety of microorganisms. Tryptic Soy Broth conforms to Harmonized USP/EP/JP requirements and is not intended for use in the diagnosis of disease or other conditions in humans.Tryptic Soy Broth, a general purpose medium, is commonly referred to as Casein Soya Bean or Soybean-Casein Digest broth, and abbreviated as TSB. This medium was originally developed for use without blood in determining the effectiveness of sulfonamides against pneumococci and other organisms. Clostridia and non-sporulating anaerobes grow luxuriantly in this broth when incubated under anaerobic conditions. TSB is recommended for testing bacterial contaminants in cosmetics and complies with established standards in the food industry.For more information visit https://www.neogen.com/solutions/Microbiology/tryptic-soy-broth
NEOGEN m-Broth, 10 kg
M Broth is used for the cultivation of Salmonella spp in a laboratory setting. M-Broth is not intended for use in the diagnosis of disease or other conditions in humans. M-Broth is prepared according to the formula of Sperber and Diebel, and contains the nutrients necessary for good growth and flagella development of Salmonella. This medium is used for cultivating Salmonella in foods and feeds by the accelerated enrichment serology (ES) procedure. M-Broth conforms to standard procedures recommended in food testing and monoclonal and polyclonal enzyme immunoassays (EIA).For more information visit https://www.neogen.com/solutions/Microbiology/m-broth
NEOGEN Brilliant Green Agar with Sulfapyridine, 500 g
Brilliant Green Agar w/ Sulfapyridine is used for the selective enrichment of Salmonella spp. in a laboratory setting. Brilliant Green Agar w/ Sulfapyridine is not intended for use in the diagnosis of disease or other conditions in humans.Brilliant Green Agar was first described by Kristensen et al and later modified by Kauffmann. The outstanding selectivity of this medium permits the use of moderately heavy inocula evenly distributed over the surface. The addition of sulfonamides into Brilliant Green Agar further inhibits Escherichia coli and Proteus spp. Osborne and Stokes used 0.1% Sodium Sulfapyridine to enhance the recovery of Salmonella from whole egg and egg yolk.For more information visit https://www.neogen.com/solutions/Microbiology/brilliant-green-agar-with-sulfapyridine
NEOGEN Buffered Peptone Water (BPW), 10 kg
Buffered Peptone Water (BPW) is used for the non-selective pre-enrichment of Salmonella spp. from food and is not intended for use in the diagnosis of disease or other conditions in humans. Edel and Kamelmacher found that food preservation techniques involving heat, desiccation, preservatives, high osmotic pressure, or pH changes cause sublethal injury to Salmonella spp. Preenrichment in a non-selective medium allows for repair of cell damage and facilitates the recovery of Salmonella. Lactose Broth is frequently used for this purpose, but it may be detrimental to recovering Salmonellae. Buffered Peptone Water maintains a high pH over the pre-enrichment period and allows in repair of injured cells that may be sensitive to low pH. This is particularly important for vegetable specimens which have a low buffering capacity. Buffered Peptone Water is used in standard methods. For more information visit https://www.neogen.com/solutions/Microbiology/buffered-peptone-water
NEOGEN Phenylethanol Agar, 500 g
Phenylethanol Agar is used with blood for the selective isolation of Gram-positive cocci in a laboratory setting. Phenylethanol Agar is not intended for use in the diagnosis of disease or other conditions in humans. Brewer and Lilley reported the addition of phenylethanol to a nutritive medium permitted growth of Gram-positive organisms, but markedly to completely inhibited growth of Gram-negative organisms. Phenylethanol Agar inhibits swarming of Proteus spp., and can be used to selectively isolate anaerobic bacteria from specimens with mixed flora. Phenylethanol Agar is specified for use in several reference methods.For more information visit https://www.neogen.com/solutions/Microbiology/phenylethanol-agar
NEOGEN OGYE Agar Base 5 kg
OGYE Agar Base is used with oxytetracycline in the detection and isolation of yeasts and molds from foods in a laboratory setting. OGYE Agar Base is not intended for use in the diagnosis of disease or other conditions in humans. Mossel, Visser, and Mengerink described Oxytetracycline-Glucose Yeast Extract (OGYE) Agar for the detection and enumeration of yeasts and molds in foods and other specimens of sanitary significance. Mossel et al. described improved recovery of yeasts and molds compared to acidified agar. This medium is supplemented with oxytetracycline as a selective agent, rather than relying on the low pH of acidified agar to suppress bacterial growth. Acidified agar is commonly used for detection of yeasts and molds in foods and dairy products, and OGYE Agar Base provides an alternate medium. For more information visit https://www.neogen.com/solutions/Microbiology/ogye-agar-base
NEOGEN Buffered Sodium Chloride - Peptone Broth pH 7.0, 10 kg
Buffered Sodium Chloride-Peptone Broth pH 7.0 is the diluent specified by the Harmonized Pharmacopoeia for the microbiological examination of non-sterile products andconforms to Harmonized USP/EP/JP Requirements in a laboratory setting. Buffered Sodium Chloride-Peptone Broth pH 7.0 is not intended for use in the diagnosis of disease or other conditions in humans.Buffered Sodium Chloride-Peptone Broth pH 7.0 is recommended by the Harmonized Pharmacopoeia (USP/EP/JP) for the microbiological examination of non-sterile products. The low level of peptone lessens the physiological shock experienced by microorganism when suspended in a diluent. The dual phosphate components create a buffered environment and sodium chloride maintains the osmotic balance.For more information visit https://www.neogen.com/solutions/Microbiology/buffered-sodium-chloride-peptone-broth-ph-7
NEOGEN Letheen Broth Base without Tween, 5 kg
Letheen Broth Base (no tween) is used with Tween 80 (Polysorbate 80) for the testing of samples containing quaternary ammonium compounds for antimicrobial activity in a laboratory setting. Letheen Broth Base (no tween) is not intended for use in the diagnosis of disease or other conditions in humans.In 1948, Weber and Black described the value of a highly nutritional solid medium containing neutralizing agents for quaternary ammonium compounds in sanitizers. The addition of Lecithin and Polysorbate 80 to Tryptone Glucose Extract (TGE) Agar resulted in a medium that effectively neutralizes quaternary ammonium compounds while testing germicidal activity. Letheen Agar is a modification of TGE Agar with the addition of Lecithin and Polysorbate 80.Letheen Broth Base was developed as a subculture medium for the neutralization of quaternary ammonium compounds in disinfectant testing.For more information visit https://www.neogen.com/solutions/Microbiology/letheen-broth-base-without-tween
NEOGEN Sabouraud Dextrose Agar, 5 kg
Sabouraud Dextrose Agar is used for the cultivation of fungi. Conforms to Harmonized USP/EP/JP performance requirements and is not intended for use in the diagnosis of disease or other conditions in humans. A medium recommended by the Harmonized Pharmacopeia for the isolation and identification of Candida albicans from non-sterile samples. Conforms to USP/EP/JP performance specification. The medium is commonly abbreviated to SDA. The medium is also used for the preparation and maintenance of fungal test strains as described by the Harmonized Pharmacopeia. The peptone digests and dextrose provide a nutritious base for luxuriant fungal growth and the acidic pH affords selectivity against bacteria. Due to the high carbohydrate content and low pH this medium is highly sensitive to overheating which will cause a drop in the gel strength.For more information visit https://www.neogen.com/solutions/Microbiology/sabouraud-dextrose-agar
NEOGEN Tryptone Glucose Extract (TGE) Agar, 500 g
Tryptone Glucose Extract (TGE) Agar is used for the cultivation and enumeration of microorganisms in dairy and bottled water products. Tryptone Glucose Extract (TGE) Agar is not intended for use in the diagnosis of disease or other conditions in humans. In the 1930’s, Bower and Hucker developed a medium for detecting bacteria in milk and other dairy products. Prickett used a glucose agar containing tryptone to study thermophilic bacteria in milk. This medium is known as Yeast Dextrose Agar. In 1948, the American Public Health Association (APHA) adopted Tryptone Glucose Extract Agar for use in testing milk and dairy products. Currently, APHA specifies Tryptone Glucose Extract Agar for the heterotrophic plate count procedure in testing bottled water. Tryptone Glucose Extract Agar is also known as Yeast Dextrose Agar.For more information visit https://www.neogen.com/solutions/Microbiology/tryptone-glucose-extract-tge-agar